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| The Background Fluorescence Monitoring task will involve the monitoring of resurgence points and streams in the study area for background concentrations of dye from previous dye
traces, pollution, and natural interference. As part of this effort, local, state, and federal agencies will be contacted to inquire whether they have recently conducted, are conducting, or plan to conduct a dye trace study in the study area or any area
potentially influencing the study area. The results of the monitoring will be used to evaluate the appropriate dyes and dye concentrations to be used if the dye tracer investigation is needed. Dye receptors will be placed at or shortly after the time of the hydrogeologic inventory and will be retrieved after a period of approximately one week. Because of background fluctuation, particularly in urban and/or industrial areas, it may be necessary to measure background weekly over a two or three week period. All monitoring activities will be conducted in accordance with the Dye Receptor Deployment and Retrieval Procedures and the Laboratory Quality Assurance/Quality Control Plan. The receptors will consist of small packets constructed of fiberglass screen mesh containing activated coconut charcoal. The receptors will be secured in the main flow of the stream or resurgence point using a system of floaters and sinkers as needed with black nylon twine (for low visibility). In small springs or streams, the channel may be altered by moving rocks or by other minor means in order to maximize flow past the receptor. In shallow water, the receptors may be shielded to minimize photochemical decay of dyes in the sunlight. Receptors will be secured so that they can be retrieved under high water conditions. Upon retrieval, each dye receptor will be washed off in the stream, placed in a clearly labeled, sealable, plastic bag, and stored in a cooler to reduce exposure to sunlight. The receptors will be delivered to the analytical laboratory (Crawford Hydrology Laboratory) for analysis. The receptors will be prepared in accordance with the Laboratory QA/QC plan and analyzed for the presence of dyes that may be used during the investigation. The dyes most often used by Crawford Hydrology Laboratory are: |
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| · Fluorescein (Acid Yellow 73) · Sulphorhodamine B (Acid Red 52) · Eosine (Acid Red 87) · Tinopal CBS-X (Fabric brightening agent 351) · D&C Red #28 · FD&C Red #3 · Rhodamine WT (Acid Red 388) · Solophenol 500% (Direct Yellow 96) · D&C Green #8 · Palanil Luminous Red G |
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