The Dye Tracing Pages

Comments on Dye Quantification for Actived Charcoal Dye Receptors

Dye concentrations are expressed in ppb. Although the dye concentration in water samples expressed in ppb is an accurate quantitative measurement of the amount of dye in the stream at the time the sample was collected, the same is not true for the eluted charcoal samples. It is only semi-quantitative compared with the actual quantity of dye in the water passing over the receptor. The quantity of dye absorbed by the charcoal is a function of the dye concentration in the water and the quantity, velocity, temperature and duration of exposure. Turbidity and the quantity and species of molecules competing with the dye for the charcoal acceptor sites can reduce the quantity of dye absorbed onto the charcoal. Also, the quantity of dye eluted from the charcoal is dependent on the amount of charcoal and eluent used, the type of eluent, whether the charcoal is wet or dry before elution, and the length of time the charcoal is eluted before being analyzed. The laboratory procedures can be standardized but the exposure variabilities while the receptor is in the stream cannot be. Although, dye concentrations for eluted samples are measured and recorded in ppb, these values will virtually always be much higher than the dye concentrations ever reached in the stream. Also, because of the several water exposure variables, the concentration of dye absorbed by the charcoal does not even accurately represent the quantity of dye that flowed in the stream past the dye receptor. Analysis of two dye receptors placed in the same general area of the same stream for the same time period will often result in differences when expressed in ppb. Therefore, the following abbreviations are used to express the interpretation of the dye concentration in more general terms rather than ppb:


ND Below Quantitation Limit
+ Positive
++ Very Positive
+++ Extremely Positive
B Background Levels
NS Receptor Not Recovered
Although dye concentrations obtained from charcoal dye receptors do not accurately reflect the concentration in the source water, detection of dye at a sufficient concentration above background levels does constitute a positive trace. If a quantitative dye trace is necessary, it must be based on dye analysis of water samples, not charcoal. Crawford Hydrology Laboratory frequently performs quantitative traces by collecting water samples with an ISCO automatic water sampler. This method provides a dye breakthrough curve, which is an accurate measurement of the dye concentration in the stream as the dye cloud passes the monitoring site.


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