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| FIELD QUALITY CONTROL SAMPLES Field Duplicates - Duplicate dye receptors are placed at 20 percent or more of the sites to be monitored. The second receptor serves as a back-up in the event that the primary receptor is lost or stolen. The secondary receptors are analyzed as duplicates for at least 10 percent of the total dye receptor locations. Trip Blanks - At least two trip blanks are prepared in the laboratory and sent along with the new dye receptors to the field. The trip blanks are then kept with the old dye receptors as they are being collected in the field, returned to the laboratory, and analyzed. LABORATORY QUALITY CONTROL SAMPLES Eluent Blank (for eluted samples only)- Each batch of eluent solution is analyzed for each dye before it is used to elute charcoal samples. Additionally, an eluent blank is analyzed at the beginning, end and every 20th sample throughout the analysis. Distilled Water Blank (for water samples only)- Each batch of distilled water is analyzed for each dye before it is used. Additionally, a distilled water blank is analyzed at the beginning, end and every 20th sample throughout the analysis. Charcoal Blank- A sample from each new sealed container of activated charcoal is eluted and analyzed for each dye before the remainder of the charcoal in the container is used. While this type of charcoal does possess fluorescence characteristics, the peaks do not coincide with the peaks for Fluorescein, Eosine, Rhodamine WT, Sulphorhodamine B, Lissamine, Tinopal CBS-X or Direct Yellow 96 in Smart solution. Raman Scattering Sample- A Raman scattering pattern and signal-to-noise ratio check is performed on the distilled water blank at the beginning and end of each sample set analysis. This is the method recommended by the manufacturer for insuring that the instrument is working within specified parameters and for calibrating the instrument. Laboratory Control Standards- A low concentration standard, at the quantitation limit for each of the dyes to be analyzed, is analyzed before and after each set of samples. This demonstrates that the Shimadzu is capable of detection at the minimum detection limit and provides data that can be used to determine the accuracy and precision of the analysis. One low concentration standard is also analyzed after every 20 samples. |
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